Evolutionary Implication of Outer Me

Evolutionary Implication of Outer Membrane Lipoprotein-Encoding Genes ompL1, lipL32 and lipL41 of Pathogenic Leptospira

Leptospirosis is recognized as the most widespread zoonosis with a global distribu-tion. In this study, the antigenic variation in Leptospira interrogans and Leptospira borgpetersenii isolated from human urine and field rat kidney was preliminarily confirmed by microscopic agglutination test using monoclonal antibodies, and was further subjected to amplification and identification of outer membrane lipopro-teins with structural gene variation. Sequence similarity analysis revealed that these protein sequences, namely OmpL1, LipL32 and LipL41, showed no more ho-mologies to outer membrane lipoproteins of non-pathogenic Leptospira and other closely related Spirochetes, but showed a strong identity within L. interrogans, suggesting intra-specific phylogenetic lineages that might be originated from a common pathogenic leptospiral origin. Moreover, the ompL1 gene showed more antigenic variation than lipL32 and lipL41 due to less conservation in secondary structural evolution within closely related species. Phylogenetically, ompL1 and lipL41 of these strains gave a considerable proximity to L. weilii and L. santaro-sai. The ompL1 gene of L. interrogans clustered distinctly from other pathogenic and non-pathogenic leptospiral species. The diversity of ompL genes has been an-alyzed and it envisaged that sequence-specific variations at antigenic determinant sites would result in slow evolutionary changes along with new serovar origina-tion within closely related species. Thus, a crucial work on effective recombinant vaccine development and engineered antibodies will hopefully meet to solve the therapeutic challenges.

作 者： K. Vedhagiri K. Natarajaseenivasan P. Chellapandi S.G. Prabhalaran Joseph Selvin S. Sharma P. Vijayachari   作者單位： K. Vedhagiri,K. Natarajaseenivasan,S.G. Prabhalaran,Joseph Selvin(Division of Medical Microbiology, Department of Microbiology, School of Life Sciences, Bharathidasan Uni-versity, Tiruchirappalli 620024, Tamilnadu, India)

P. Chellapandi(Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India)

S. Sharma,P. Vijayachari(Regional Medical Research Centre RMRC, Indian Council of Medical Research / WHO Collaborative Centre for Diagnosis, Research, Ref-erence and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India) 

刊 名： 基因組蛋白質(zhì)組與生物信息學(xué)報(bào)（英文版）  英文刊名： GENOMICS、PROTEOMICS & BIOINFORMATICS  年，卷(期)： 2009 7(3)  分類號(hào)： Q5  關(guān)鍵詞： Leptospira   ompL1   lipL32   lipL41   phylogeny   antigenic variation  

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